Shyamtanu Chattoraj


Ph.D. in Physical Chemistry, IACS-Kolkata, India

shyamtanu_chattoraj {aaattt}hms{ddoott}harvard{ddoott}edu

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Three-dimensional (3D) folding and spatial organization of chromosomes including genome positioning and organization of several sub-structures of chromatin such as chromatin domains and territories are responsible for gene expression and gene regulation. However, a proper understanding on the relationship between genome organization and genome function in diseased cells is still lacking. Our current understanding on chromatin structure has been hindered by the diffraction limit of light (250 nm), which falls far short of enabling us to visualize chromatin structure. Recently, Wu lab has developed a pioneering technique called OligoSTORM, which circumvents this limitation and enables visualization of chromatin at unprecedented spatial resolution, at the nanoscale (<20nm resolution). Here my goal is to investigate the 3D spatial organization and packaging of chromosomal territories of trisomy 21 in the nucleus of Down syndrome (DS) cells, using a combination of a microscopy, including OligoSTORM, sequencing-based approaches, such as chromosome conformation capture (Hi-C), as well as chromatin modeling. I want to explore correlations among chromosome organization, patterns of gene positioning, and disease phenotypes in the context of Down syndrome pathology and therapeutics. I will be asking, how does the aneuploid addition of genomic material affect the organization of the genome? Does the imbalance of gene expression caused by aneuploidy correspond to changes in genomic structure? Since I will be using imaging technologies that have not been widely or at all implemented for imaging aneuploid genomes, I hope to open new avenues of investigation. The results of these projects may provide new insights into the nano-architectures of the chromosomal abnormalities that characterize diseased cells.


Huy Q. Nguyen, Shyamtanu Chattoraj, David Castillo, Son C. Nguyen, Guy Nir, Antonios Lioutas, Elliot A. Hershberg, Nuno M. C. Martins, Paul L. Reginato, Mohammed Hannan, Brian J. Beliveau, George M. Church, Evan R. Daugharthy, Marc A. Marti-Renom & C.-ting Wu. Nat Methods 17, 822–832 (2020).

Walking along chromosomes with super-resolution imaging, contact maps, and integrative modeling.

Guy Nir, Irene Farabella, Cynthia Pérez Estrada, Carl G. Ebeling, Brian J. Beliveau, Hiroshi M. Sasaki, S. Dean Lee, Son C. Nguyen, Ruth B. McCole, Shyamtanu Chattoraj, Jelena Erceg, Jumana AlHaj Abed, Nuno M. C. Martins, Huy Q. Nguyen, Mohammed A. Hannan, Sheikh Russell, Neva C. Durand, Suhas S. P. Rao, Jocelyn Y. Kishi, Paula Soler-Vila, Michele Di Pierro, José N. Onuchic, Steven P. Callahan, John M. Schreiner, Jeff A. Stuckey, Peng Yin, Erez Lieberman Aiden, Marc A. Marti-Renom, C.-ting Wu. PLOS Genetics, December 26, 2018

Differential role of nonmuscle myosin II isoforms during blebbing of MCF-7 cells.

Dey, S.K., Singh, R. K., Chattoraj, S., Saha, S., Das, A., Bhattacharyya, K., Sengupta, K., Sen, S., Jana, S. S.

Mol. Biol. Cell 2017.

Structural oscillations of non-muscle myosin II-C2: time resolved confocal microscopy.

Chattoraj, S. , Saha, S., Halder, D., Jana, S. S., Bhattacharyya, K. 2017

ChemistrySelect. 2:953-958.

Biological oscillations: Fluorescence monitoring by confocal microscopy.

Chattoraj, S. , Bhattacharyya, K. 2017.

Chem. Phys. Letts. 660:1-10

Cytochrome c-capped fluorescent gold nanoclusters: Imaging of live cells and delivery of Cytochrome c.

Chattoraj, S., Amin, M.A., Bhattacharyya, K. 2016.

ChemPhysChem. 17:2088-2095.

Selective killing of breast cancer cells by doxorubicin loaded fluorescent gold nanoclusters: Confocal microscopy and FRET.

Chattoraj, S., Amin, M.A., Jana, B., Mohapatra, S., Ghosh, S., Bhattacharyya, K. 2016.

Chem.Phys.Chem. 17:253-259

Cancer cell imaging using in situ generated gold nanoclusters.

Chattoraj, S., Amin, M.A., Mohapatra, S., Ghosh, S., Bhattacharyya, K. 2016.

Chem.Phys.Chem. 17:61-68

Direct observation of the growth and shrinkage of microtubules by single molecule Förster resonance energy transfer.

Mondal, P., Chattoraj, S., Chowdhury, R., Bhunia, D., Ghosh, S., Bhattacharyya, K. 2015.

Phys. Chem. Chem. Phys. 17:6687-6690

Role of red-ox cycle in structural oscillations and solvation dynamics in the mitochondria of a live cell.

Chattoraj, S., Chowdhury, R., Dey, S.K., Jana, S.S., Bhattacharyya, K. 2015.

J. Phys. Chem. B 118: 22339-22346

Dynamics of gene silencing in a live cell: stochastic resonance.

Chattoraj, S., Saha, S., Jana, S.S., Bhattacharyya, K. 2014.

J. Phys. Chem. Lett. 5:1012-1016

Effect of ethanol-water mixture on the structure and dynamics of lysozyme: A fluorescence correlation spectroscopy study.

Chattoraj, S., Mandal, A.K., Bhattacharyya, K. 2014.

J. Chem. Phys. 118: 22339-22346

Dynamics in cytoplasm, nucleus, and lipid droplet of a live CHO cell: time-resolved confocal microscopy.

Ghosh, S., Chattoraj, S., Mondal, T., Bhattacharyya, K. 2013.

Langmuir 29:7975-7982