Huy Nguyen


Ph.D. in Genetics, Dartmouth College



The 3D organization of the genome plays a critical role in cellular processes including gene expression. Unfortunately, the diffraction limit of light has constrained our ability to achieve a complete understanding of fine scale details of organization. A new technology developed by the Wu lab, called OligoSTORM, circumvents this limitation and enables visualization of chromatin at unprecedented levels, at the nanoscale (<20nm resolution). My research uses OligoSTORM to uncover chromatin nanostructure in human cells. Furthermore, I study the effects of extreme stress, including zero-gravity and desiccation, on chromatin nanostructure. My interests stem from improving human health, both on Earth and in Space. An understanding of chromatin nanostructure and the impact that extreme stresses may have on it is critical, given the role that organization plays on transcription. Furthermore, my research aims to develop ways in which we can better protect our genomes.


Walking along chromosomes with super-resolution imaging, contact maps, and integrative modeling.

Guy Nir, Irene Farabella, Cynthia Pérez Estrada, Carl G. Ebeling, Brian J. Beliveau, Hiroshi M. Sasaki, S. Dean Lee, Son C. Nguyen, Ruth B. McCole, Shyamtanu Chattoraj, Jelena Erceg, Jumana AlHaj Abed, Nuno M. C. Martins, Huy Q. Nguyen, Mohammed A. Hannan, Sheikh Russell, Neva C. Durand, Suhas S. P. Rao, Jocelyn Y. Kishi, Paula Soler-Vila, Michele Di Pierro, José N. Onuchic, Steven P. Callahan, John M. Schreiner, Jeff A. Stuckey, Peng Yin, Erez Lieberman Aiden, Marc A. Marti-Renom, C.-ting Wu. PLOS Genetics, December 26, 2018

Gene Positioning Effects on Expression in Eukaryotes.

Nguyen HQ and Bosco G.

Annu Rev Genet. 2015 Oct 2. DOI: 10.1146/annurev-genet-112414-055008.

Widespread Rearrangement of 3D Chromatin Organization Underlies Polycomb-Mediated Stress-Induced Silencing.

Li Li, Lyu X, Hou C, Takenaka N, Nguyen HQ, Ong CT, Cubenas-Potts C, Hu M, Lei E, Bosco G, Qin Z, Corces V.

Mol Cell. 2015 Mar 26. Doi:10.1016/j.molcel.2015.02.023.

Drosophila Casein Kinase I Alpha Regulates Homolog Pairing and Genome Organization by Modulating Condensin II Subunit.

Nguyen HQ, Nye J, Buster DW, Klebba JE, Rogers GC, Bosco G. .

PLoS Genet. 2015 Feb 27. Doi:10.1371/journal.pegen.1005014.

Condensins Exert Force on Chromatin-Nuclear Envelope Tethers To Mediate Nucleoplasmic Reticulum Formation in Drosophila melanogaster.

Bozler J, Nguyen HQ, Rogers GC, Bosco G.

G3 (Bethesda). 2014 Dec 30. pii: g3.114.015685. doi: 10.1534/g3.114.015685.

Condensins are Required for Maintenance of Nuclear Architecture.

George CM, Bozler J, Nguyen HQ, Bosco G.

Cells. 2014 Aug 22;3(3):865-82. doi: 10.3390/cells3030865.

Novel diet-related mouse model of colon cancer parallels human colon cancer.

Prasad AR, Prasad S, Nguyen HQ, Facista A, Lewis C, Zaitlin B, Bernstein H, Bernstein C.

Oncol. 2014 Jul 15;6(7):225-43. Doi:10.4251/wjgo.v6.i7.225.

Maintenance of interphase chromosome compaction and homolog pairing in Drosophila is regulated by the condensin Cap-h2 and its partner Mrg15.

Smith HF, Roberts MA, Nguyen HQ, Peterson M, Hartl TA, Wang XJ, Klebba JE, Rogers GC, Bosco G.

Genetics. 2013 Sep;195(1):127-46. doi: 10.1534/genetics.113.153544.

SCFSlimb ubiquitin ligase suppresses condensin II-mediated nuclear reorganization by degrading Cap-H2.

Buster DW, Daniel SG, Nguyen HQ, Windler SL, Skwarek LC, Peterson M, Roberts M, Meserve JH, Hartl T, Klebba JE, Bilder D, Bosco G, Rogers GC.

J Cell Biol. 2013 Apr 1;201(1):49-63. doi: 10.1083/jcb.201207183.

Deficient expression of DNA repair enzymes in early progression to sporadic colon cancer.

Facista A, Nguyen HQ, Lewis C, Prasad AR, Ramsey L, Zaitlin B, Nfonsam V, Krouse RS, Bernstein H, Payne CM, Stern S, Oatman N, Banerjee B, Bernstein C.

Genome Integr. 2012 Apr 11.

MCPH1 Negative Regulation of Condensin II Ensures Proper Mitosis and Interphase Genome Organization.

Nguyen HQ, McLammarah T, Kacsoh BZ, Hodge S, Rogers GC, Bosco G.

2016. In Prep